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RNA Seq
•
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0
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9
replies
3.2k
views
How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 4.1 years ago by
bernatgel
▴ 150 • written 5.1 years ago by
adeler001
• 0
2
votes
5
replies
3.8k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 4.6 years ago by
Steve Lianoglou
★ 13k • written 4.6 years ago by
svlachavas
▴ 840
0
votes
3
replies
908
views
Normalization of RNA seq data
RNA seq
cpm
updated 4.6 years ago by
Kevin Blighe
★ 4.0k • written 4.6 years ago by
isabel.pieterse
• 0
4
votes
4
replies
1.1k
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 3 months ago by
Sara
• 0 • written 5.0 years ago by
116100016
• 0
1
vote
4
replies
1.1k
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 5.0 years ago by
James W. MacDonald
67k • written 5.0 years ago by
abf
▴ 30
0
votes
0
replies
646
views
searching for specific RNA sequences
rna seq
5.0 years ago
adeler001
• 0
0
votes
2
replies
969
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 5.1 years ago by
Leonard Goldstein
▴ 260 • written 5.1 years ago by
116100016
• 0
0
votes
1
reply
667
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 5.2 years ago by
Michael Love
43k • written 5.2 years ago by
adeler001
• 0
0
votes
2
replies
1.1k
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 5.3 years ago by
Michael Love
43k • written 5.3 years ago by
adeler001
• 0
1
vote
5
replies
1.5k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
mahes.muniandy
• 0
5
votes
3
replies
1.2k
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 5.5 years ago by
Peter Langfelder
★ 3.0k • written 5.5 years ago by
agustin.gonvi
▴ 20
1
vote
2
replies
2.2k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
5.6 years ago
Mozart
▴ 30
0
votes
0
replies
1.8k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
6.8 years ago
lawarde.ankita1
▴ 30
13 results • Page
1 of 1
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Replies
Comment: Crossover design with limma
by
SamGG
▴ 360
IMHO, here are the missing annotations treatmentdrug_orderDrugPlac - treatmentplacebo_orderDrugPlac, # effect of drug for the Drug…
Answer: What type of counts data to import for performing Isoform analysis in edgeR
by
ATpoint
★ 4.5k
You don't need any of these files. You need the `catchSalmon` function which reads `quant.sf` and the inferential (Bootstrap/Gibbs) replica…
Comment: makeContrastsDream for Interaction
by
abadgerw
• 0
Thank you! I will try this out.
Comment: Merge Replicates vs Mixed Model
by
abadgerw
• 0
Thanks, @gabrielhoffman The data is actually proteomics so it is intensity data which is why I did not use voom but rather ran ebayes wi…
Comment: ANOVA like approach of edgeR
by
Gordon Smyth
52k
To identify common upregulated or downregulated genes, you would need to test for media condition contrasts for each cell type separately, …
Votes
Comment: Crossover design with limma
Answer: DESeq2 normalization vs VST vs rlog
Comment: makeContrastsDream for Interaction
Answer: Limma- Print-tip loess normalisation-printer layout information does not match M
Answer: Limma- Print-tip loess normalisation-printer layout information does not match M
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